Wu culture has the characteristics of obvious regional color of water town and distinctive characteristics of citizen culture. The shape and cutting of Jiangnan women's folk costumes all reflect the characteristics of Wu culture. Jiangnan folk women's costumes reproduce Jiangnan folk culture, which is helpful to improve women's status, inherit the spirit of craftsmanship and contain social and cultural values. The research on Jiangnan women's folk costumes under the background of Wu cultural heritage protection is conducive to promoting the protection and utilization of cultural heritage in Jiangnan area.